Introduction:

Brucellosis, is a zoonotic systemic infectious disease caused by Brucella bacteria. Infected non-pregnant female animals typically show no apparent clinical symptoms, while the most significant symptom in pregnant female animals infected with Brucella is abortion.

This assay is designed to detect antibodies against Brucella (BRU) in serum or plasma of Ovine/Caprine. It can be used for the evaluation of the immunological efficacy of the BRU vaccine or as an adjunctive diagnostic tool for the disease.

Principle :

This kit comprises a coated Microtiter Plate with BRU antigen, HRP conjugate, and other accompanying reagents. It employs the principle of enzyme-linked immunosorbent assay (ELISA) to detect antibodies against BRU in the serum or plasma of Ovine/Caprine. During the experiment, control serum and samples are added to the plate. After incubation, if the sample contains BRU antibodies, they will bind to the antigens coated on the microtiter plate. Following washing steps to remove unbound components, the HRP conjugate is added, which specifically binds to the antigen-antibody complexes on the plate. After washing again to remove unbound HRP conjugate, substrate reagents are added to the wells and react with the enzyme-labeled complexes, resulting in a blue color. The intensity of the color is directly proportional to the amount of specific antibody present in the sample. The reaction is then terminated by adding a stop solution, turning the solution yellow. The absorbance of each well is measured at a wavelength of 450nm using a microtiter plate reader (microplate reader) to determine the presence of BRU antibodies in the sample.