Introduction:

Avian influenza, also known as European fowl plague, is an acute and highly lethal contagious disease affecting birds, caused by Influenza A virus. It is characterized by acute septicemic death or asymptomatic carrier status.

This assay is designed to detect avian influenza virus (AIV) antibodies in the serum, plasma, or egg yolk of avian species including chickens, ducks, and geese. It can be employed for the assessment of immunization efficacy and as a supportive diagnostic tool.

Principle :

This kit comprises a coated Microtiter Plate with AIV antigen, antibody solution, HRP conjugate, and other accompanying reagents. It employs the principle of competitive enzyme-linked immunosorbent assay to detect antibodies against the AIV in the serum, plasma, or egg yolk of avian species. During the experiment, diluted samples and antibody solution are added to the plate. If the sample contains AIV antibodies, they will compete with the antibody solution to bind with the antigen on the plate, thereby preventing subsequent color development. Conversely, color development occurs in the absence of such antibodies. The intensity of the color is inversely proportional to the specific antibody content in the sample. After adding stop solution to terminate the reaction, the product turns yellow. The absorbance values in each well are measured at a wavelength of 450nm using an microtiter plate reader (microplate reader), allowing determination of the presence of AIV antibodies in the sample.