Introduction:

Toxoplasmosis is a zoonotic parasitic disease caused by Toxoplasma gondii (TOX). Its primary characteristics include fever, rapid breathing, drowsiness, and hindlimb paralysis.

This kit is designed for the detection of antibodies against TOX in porcine serum or plasma and can be used to assess the TOX infection status in pig farms.

Principle :

This kit comprises a coated Microtiter Plate with TOX recombinant antigens, HRP conjugate, and other accompanying reagents. It employs the principle of enzyme-linked immunosorbent assay (ELISA) to detect TOX antibodies in the porcine serum or plasma. During the experiment, control serum and test sample are added to the plate. After incubation, if the sample contains TOX antibodies, they will bind to the antigens coated on the microtiter plate. Following washing steps to remove unbound components, the HRP conjugate are added, which specifically binds to the antigen-antibody complexes on the plate. After washing again to remove unbound HRP conjugate, substrate reagents are added to the wells and react with the enzyme-labeled complexes, resulting in a blue color. The intensity of the color is directly proportional to the amount of specific antibody present in the sample. The reaction is then terminated by adding a stop solution, turning the solution yellow. The absorbance of each well is measured at a wavelength of 450nm using a microtiter plate reader (microplate reader) to determine the presence of TOX antibodies in the sample.