Introduction:

Porcine Japanese encephalitis is a mosquito-borne, acute zoonotic infectious disease caused by the Porcine Japanese Encephalitis Virus (JEV). Infected pigs primarily exhibit symptoms such as abortions and stillbirths in pregnant sows, and testicular swelling in boars.

This assay is designed to detect antibodies against Japanese Encephalitis Virus (JEV) in porcine serum or plasma. It can be used to evaluate the immunological efficacy of the JEV vaccine.

Principle :

This kit comprises a coated Microtiter Plate with recombinant E2 protein of porcine JEV, HRP conjugate, and other accompanying reagents. It employs the principle of enzyme-linked immunosorbent assay (ELISA) to detect antibodies against JEV in the porcine serum or plasma. During the experiment, control serum and test sample are added to the plate. After incubation, if the sample contains JEV antibodies, they will bind to the antigens coated on the microtiter plate. Following washing steps to remove unbound components, the HRP conjugate is added, which specifically binds to the antigen-antibody complexes on the plate. After washing again to remove unbound HRP conjugate, substrate reagents are added to the wells and react with the enzyme-labeled complexes, resulting in a blue color. The intensity of the color is directly proportional to the amount of specific antibody present in the sample. The reaction is then terminated by adding a stop solution, turning the solution yellow. The absorbance of each well is measured at a wavelength of 450nm using a microtiter plate reader (microplate reader) to determine the presence of antibodies against JEV in the sample.