Introduction:

Pseudorabies, also known as Aujeszky's disease, is an acute infectious disease of livestock and various wild animals characterized by fever, pruritus, respiratory and neurological disorders. It is caused by the Pseudorabies Virus (PRV).

This assay is designed to detect antibodies against pseudorabies virus glycoprotein D (PRV-gD) in porcine serum or plasma. It can be used for the evaluation of the immunological efficacy of the pseudorabies virus vaccine in pigs.

Principle :

This kit comprises a coated Microtiter Plate with PRV gD protein, HRP conjugate, and other accompanying reagents. It employs the principle of enzyme-linked immunosorbent assay (ELISA) to detect antibodies against PRV gD in the porcine serum or plasma. During the experiment, control serum and test sample are added to the plate. After incubation, if the sample contains PRV gD antibodies, they will bind to the antigens coated on the microtiter plate. Following washing steps to remove unbound components, the HRP conjugate is added, which specifically binds to the antigen-antibody complexes on the plate. After washing again to remove unbound HRP conjugate, substrate reagents are added to the wells and react with the enzyme-labeled complexes, resulting in a blue color. The intensity of the color is directly proportional to the amount of specific antibody present in the sample. The reaction is then terminated by adding a stop solution, turning the solution yellow. The absorbance of each well is measured at a wavelength of 450nm using a microtiter plate reader (microplate reader) to determine the presence of PRV gD antibodies in the sample.