Introduction:

Swine fever, caused by the Classical Swine Fever Virus (CSFV), is a highly contagious, hemorrhagic, and lethal disease in pigs. It is characterized by prolonged high fever and degeneration of the microvascular walls leading to extensive hemorrhage, necrosis, and thrombosis. The chronic form is characterized by fibro-necrotic enteritis.

This assay is designed to detect Classical Swine Fever Virus (CSFV) antibodies in porcine serum or plasma. It can be employed for the assessment of immunization efficacy.

Principle :

This kit comprises a coated Microtiter Plate with CSFV E2 protein, HRP conjugate, and other accompanying reagents. It employs the principle of enzyme-linked immunosorbent assay(ELISA) to detect antibodies against CSFV in the porcine serum or plasma. During the experiment, control serum and test sample are added to the plate. After incubation, if the sample contains CSFV antibodies, they will bind to the antigens coated on the microtiter plate. Following washing steps to remove unbound components, the HRP conjugate is added, which specifically binds to the antigen-antibody complexes on the plate. After washing again to remove unbound HRP conjugate, substrate reagents are added to the wells and react with the enzyme-labeled complexes, resulting in a blue color. The intensity of the color is directly proportional to the amount of specific antibody present in the sample. The reaction is then terminated by adding a stop solution, turning the solution yellow. The absorbance of each well is measured at a wavelength of 450nm using a microtiter plate reader (microplate reader) to determine the presence of antibodies against CSFV in the sample.